1Faculty of Medicine of Sousse, Laboratory of Physiology, University of Sousse. Tunisia
2Faculty of P.E. and Sports Science, University of Athens, Greece
3Hospital of Farhat Hached, Biochemical Department, Sousse, Tunisia
4Higher Institute of Sport and Physical Education of Tunis, University of Manouba, Tunisia
5Faculty of Kinesiology & Physical Education, University of Toronto, Toronto, ON, Canada
Aim: was to assess the effects of Ramadan observance upon repeated sprints and associated inflammatory and oxidative stress responses. Methods: Ten young trained boxers were tested during a control period (C), at the end of the first week (R-1), and during the fourth week of Ramadan observance (R-4). On each occasion, they performed three vertical jumps, 10 x 6 s repeated sprints on a cycle ergometer, followed by three final vertical jumps 1 min after. Surface electrodes measured the EMG activity of the vastus lateralis during jumps performed before and after sprinting. Oxidative stress (malondialdehyde, total antioxidant and catalase), inflammatory markers (C-reactive protein, Interleukin-6 and homocysteine), muscle damage (CPK and LDH) and blood glucose were measured at rest and after completing the exercise protocol. Results: The overall sprint performance was reduced at R-1 compared to C (-6.3 ± 1.2%, p = 0.025), but had recovered by R-4. Jump height decreased after the repeated sprints (p<0.01), without significant changes in EMG parameters. Oxidative stress indices, inflammatory markers, and muscle damage measured after the protocol exercise were unaffected during fasting. Conclusion: The correction of sprint performance may highlight some adaptive responses to fasting. The unchanged of the selected fatigue biomarkers after the repeated sprint protocol suggest that the dietary restriction related to Ramadan is not severe enough to induce significant changes in the metabolism of our trained athletes.
Bouhlel H, Shephard RJ, Gmada N, Aouichaoui C, Peres G, Tabka Z, et al. Effect of Ramadan Observance on Maximal Muscular Performance of Trained Men. Clin J Sport Med 2013; 23(3): 222-7.
Shephard RJ. The impact of Ramadan performance upon athletic performance. Nutrients 2012; 4: 491-505.
El Ati J, Beji C, Danguir J. Increased fat oxidation during Ramadan fasting in healthy women. An adaptative mechanism for body weight maintenance. Am J Clin Nutr 1995 ; 62:302-7.
Bouhlel E, Salhi Z, Bouhlel H, Mdella S, Amamou A, Zaouali M, et al. Effect of Ramadan fasting on fuel oxidation during exercise in trained male rugby players. Diabetes & Metab 2006; 32: 617-24.
Bouhlel E, Zaouali M, Miled A, Tabka Z, Bigard X, Shephard R. Ramadan fasting and the GH/IGF-1 axis of trained men during submaximal exercise. Ann Nutr Metab 2008a; 52: 261-6.
Bouhlel E, Denguezli M, Zaouali M, Tabka Z, Shephard RJ. Ramadan fasting’s effect on plasma, leptin, adiponectin concentrations, and body composition in trained young men. Int J Sport Nutr Exerc Metab 2008b; 18:617-27.
Bishop D, Girard O, Mendez-Villanueva A. Repeated-Sprint ability-Part II. Recommendations for training. Sports Med 2011; 41(9): 741-56.
Aloui A, Chaouachi A, Chtourou H, Wong del P, Haddad M, Chamari K, et al. Effects of Ramadan on the diurnal variations of repeated-sprint performances. Int J Sports Physiol Perform 2013 ; 8(3):254-62.
Meckel Y, Ismael A, Eliakim A The effect of Ramadan fast on physical performance and dietary habits in adolescent soccer players. Eur J Appl Physiol Occup Physiol 2008; 102: 651-7.
Racinais S, Périard JD, Li CK, Grantham J. Activity patterns, body composition and muscle function during Ramadan in a Middle-East Muslim country. Int J Sports Med 2012 ; 33(8): 641-6.
Hammouda O, Chahed H, Chtourou H, Ferchichi S, Miled A, Souissi N. Morning-to-evening difference of biomarkers of muscle injury and antioxidant status in young trained soccer players, Biol. Rhythm Res 2012; 43: 431-8.
Chaouachi A, Leiper JB, Chtourou H, Aziz AR, Chamari K. The effects of Ramadan intermittent fasting on athletic performance: recommendations for the maintenance of physical fitness. J Sports Sci 2012; 30 Suppl 1:S53-73. doi: 10.1080/02640414.2012.698297.
Ibrahim WH, Habib HM, Jarrar AH, Al Baz SA. Effect of Ramadan Fasting on Markers of Oxidative Stress and Serum Biochemical Markers of Cellular Damage in Healthy Subjects. Ann. Nutr. Metab 2008; 53 (3-4):175-81.
Askungar FB, Topkaya AE, Akyildiz M. Interleukin-6, C-reactive protein and biochemical parameters during prolonged intermittent fasting. Ann Nutr Metab 2007; 51: 88-95.
Unalaçak M, Kara IH, Baltaci D, Erdem O, Bucaktepe PGE. Effects of Ramadan fasting on biochemical and haematological parameters and cytokines in healthy and obese individuals. Metab Syndr Relat Disord 2011; 2(9): 157-61.
Chennaoui M, Desgorces F, Drogou C, Boudjemaa B, Tomaszewski A, Depiesse F, Burnat P, Chalabi H, Gomez-Merino D. Effects of Ramadan fasting on physical performance and metabolic, hormonal, and inflammatory parameters in middle-distance runners. Appl Physiol Nutr Metab 2009 ; 34:587-94.
Durnin JVGA, Womersley J. Body fat assessed from total body density and its estimation from skinfold thickness: measurements on 481 men and women aged from 16 to 72 years. Br J Nutr 1974; 32: 77-97
Bar-Or, O. Wingate anaerobic test. An update on methodology, reliability and validity. Sports Med 1987; 4(6): 381-94.
Gaitanos GC, Williams C, Boobis LH, Brooks S. Human muscle metabolism during intermittent maximal exercise. J Appl Physiol 1993; 75(2): 712-9.
Glaister M, Stone MH, Stewart AM, Hughes M, Moir GL. The reliability and validity of fatigue measures during short-duration maximal-intensity intermittent cycling. J Strength Cond Res 2004; 18: 459-62.
Yagi K. A simple fluorometric assay for lipoperoxide in blood plasma. Biochem. Med. Metab 1976;15: 212-6.
Goth L. A simple method for determination of serum catalase activity and revision of reference range Clin Chim Acta 1991; 196: 143-52.
Souissi N, Souissi H, Sahli S, Tabka Z, Dogui M, Ati J, Davenne D. Effect of Ramadan on the diurnal variation in short-term high power output. Chronobiol Int 2007 ; 24(5): 991-1007.
Chaouachi A, Coutts AJ, Chamari K, Wong del P, Chaouachi M, Chtara M, Roky R, Amri M. Effect of Ramadan intermittent fasting on aerobic and anaerobic performance and perception of fatigue in male elite judo athletes. J Strength Cond Res 2009a; 23(9):2702-9
Kirkendall DT, Leiper JB, Bartagi Z, Dvorak J, Zerguini Y. The influence of Ramadan on physical performance measures in young Muslim footballers. J Sports Sci 2008; 26 Suppl 3:S15-27. doi: 10.1080/02640410802422199.
Bogdanis GC. Effects of physical activity and inactivity on muscle fatigue. Front Physiol 2012; 18;3:142. doi: 10.3389/fphys.2012.001422.
Chtourou H, Hammouda O, Chaouachi A, Chamari K, Souissi N. The effect of time-of-day and Ramadan fasting on anaerobic performances. Int J Sports Med 2012 ; 33(2):142-7.
Chtourou H, Hammouda O, Souissi H, Chamari K, Chaouachi A, Souissi N. The effect of Ramadan fasting on physical performances, mood state and perceived exertion in young footballers. Asian J Sports Med 2011; 2(3):177-85.
Faris MA, Kacimi S, Al-Kurd RA, Fararjeh MA, Bustanji YK, Mohammad MK, Salem ML. Intermittent fasting during Ramadan attenuates proinflammatory cytokines and immune cells in healthy subjects. Nutr Res 2012; 32(12): 947-55.
Chaouachi A, Coutts AJ, Wong del P, Roky R, Mbazaa A, Amri M, Chamari K. Haematological, inflammatory, and immunological responses in elite judo athletes maintaining high training loads during Ramadan. Appl. Physiol. Nutr. Metab 2009b; 34(5):907-15.
Hammouda O, Chtourou H, Aloui A, Chahed H, Kallel C, Miled A, Chamari K, Chaouachi A, Souissi N. Concomitant Effects of Ramadan Fasting and Time-Of-Day on Apolipoprotein AI, B, Lp-a and Homocysteine Responses during Aerobic Exercise in Tunisian Soccer Players. PLoS One 2013; 11;8(11).
Abbey EL, Rankin JW. Effect of quercetin supplementation on repeated-sprint performance, xanthine oxidase activity, and inflammation. Int J Sport Nutr Exerc Metab 2011; 21: 91-6.