In vitro Antimicrobial Effect of Probiotic Films Based on Carboxymethyl Cellulose-Sodium Caseinate Against Common Food-Borne Pathogenic Bacteria

Document Type : Research Paper


1 Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran


Introduction: Consumption of appropriate amount of probiotic microorganisms via food products have health benefits on the host. In recent years, there has been a significant increase in research on the characterization and verification potential use of probiotic films in food industry. The aim of the current study was to investigate in vitro antimicrobial property of probiotic carboxymethyl cellulose-sodium caseinate (CMC-SC) films containing Lactobacillus acidophilus, L. reuteri and Bifidobacterium bifidum against Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus and Escherichia coli O157:H7. Methods: Preparation of CMC-SC composite films were conducted based on casting method. The in vitro antibacterial property of CMC-SC films was evaluated using agar disk diffusion and broth micro-dilution methods. Results: Antimicrobial property of probiotic films (diameter inhibition zone and log differences in population, respectively) were as follow: S. aureus (2.13-5.65 mm and -0.79 - -3.82) > L. monocytogenes (1.76-5.32 mm and -0.65 - -3.34) > S. typhimurium (2.13-4.33 mm and -0.34 - -2.79) > E. coli O157:H7 (1.88-3.86 mm and -0.18 - -2.62). The best antimicrobial property against aforementioned bacterial pathogens was found for film supplemented with L. acidophilus + L. reuteri + B. bifidum. Conclusion: It can be concluded that incorporation of some probiotic strains into edible films resulted in excellent antimicrobial property against S. aureus, L. monocytogenes, S. typhimurium and E. coli O157:H7 and probably solve safety related issue in food industry.


1. Gialamas H, Zinoviadou KG, Biliaderis CG, Koutsoumanis KP. Development of a novel bioactive packaging based on the incorporation of Lactobacillus sakei into sodium-caseinate films for controlling Listeria monocytogenes in foods. Food Res Int. 2010; 43(10):2402-8.
2. Jay JM, Loessner MJ, Golden DA. Modern food microbiology, 7th ed.,. New York, NY: Springer Science Business Media, Inc,; 2005.
3. Barmpalia IM, Koutsoumanis KP, Geornaras I, Belk KE, Scanga JA, Kendall PA, et al. Effect of antimicrobials as ingredients of pork bologna for Listeria monocytogenes control during storage at 4 or 10 ºC. Food Microbiol. 2005; 22(2-3):205-11.
4. Fernández-Pan I, Carrión-Granda X, Maté JI. Antimicrobial efficiency of edible coatings on the preservation of chicken breast fillets. Food Control. 2014; 36(1):69-75.
5. Saad N, Delattre C, Urdaci M, Schmitter J-M, Bressollier P. An overview of the last advances in probiotic and prebiotic field. LWT-Food Sci Technol. 2013; 50(1):1-16.
6. Espitia PJ, Batista RA, Azeredo HM, Otoni CG. Probiotics and their potential applications in active edible films and coatings. Food Res Int. 2016; 90:42-52.
7. Liu L, O’Conner P, Cotter P, Hill C, Ross R. Controlling Listeria monocytogenes in cottage cheese through heterologous production of enterocin a by Lactococcus lactis. J Appl Microbiol. 2008; 104(4):1059-66.
8. Concha-Meyer A, Schöbitz R, Brito C, Fuentes R. Lactic acid bacteria in an alginate film inhibit Listeria monocytogenes growth on smoked salmon. Food Control. 2011;22(3-4):485-9.
9. Maragkoudakis PA, Mountzouris KC, Psyrras D, Cremonese S, Fischer J, Cantor MD, et al. Functional properties of novel protective lactic acid bacteria and application in raw chicken meat against Listeria monocytogenes and Salmonella enteritidis. Int J Food Microbiol. 2009; 130(3):219-26.
10. Pavli F, Kovaiou I, Apostolakopoulou G, Kapetanakou A, Skandamis P, Nychas G-J, et al. Alginate-based edible films delivering probiotic bacteria to sliced ham pretreated with high pressure processing. Int J Molecul Sci. 2017; 18(9):1867.
11. Sánchez-González L, Saavedra JIQ, Chiralt A. Antilisterial and physical properties of biopolymer films containing lactic acid bacteria. Food Control. 2014; 35(1):200-6.
12. Soukoulis C, Singh P, Macnaughtan W, Parmenter C, Fisk ID. Compositional and physicochemical factors governing the viability of Lactobacillus rhamnosus gg embedded in starch-protein based edible films. Food Hydrocoll. 2016; 52:876-87.
13. Khezrian A, Shahbazi Y. Application of nanocompostie chitosan and carboxymethyl cellulose films containing natural preservative compounds in minced camel’s meat. Int J Biol Macromol. 2018; 106:1146-58.
14. Belyamani I, Prochazka F, Assezat G. Production and characterization of sodium caseinate edible films made by blown-film extrusion. J Food Eng. 2014; 121:39-47.
15. Lacroix M, Cooksey K. Edible films and coatings from animal origin proteins.  Innovations in food packaging: Elsevier; 2005. p. 301-17.
16. Montero P, Mosquera M, Marín-Peñalver D, Alemán A, Martínez-Álvarez Ó, Gómez-Guillén MC. Changes in structural integrity of sodium caseinate films by the addition of nanoliposomes encapsulating an active shrimp peptide fraction. J Food Eng. 2019; 244:47-54.
17. Shahbazi Y. Application of carboxymethyl cellulose and chitosan coatings containing Mentha spicata essential oil in fresh strawberries. Int J Biol Macromol2018; 112:264-72.
18. Rezaei F, Shahbazi Y. Shelf-life extension and quality attributes of sauced silver carp fillet: A comparison among direct addition, edible coating and biodegradable film. LWT-Food Sci Technol. 2018; 87:122-33.
19. Shahbazi Y. The properties of chitosan and gelatin films incorporated with ethanolic red grape seed extract and Ziziphora clinopodioides essential oil as biodegradable materials for active food packaging. Int J Biol Macromol. 2017; 99:746-53.
20. Han Y, Wang L. Sodium alginate/carboxymethyl cellulose films containing pyrogallic acid: Physical and antibacterial properties. J Sci Food Agric 2017; 97(4):1295-301.
21. Saba MK, Sogvar OB. Combination of carboxymethyl cellulose-based coatings with calcium and ascorbic acid impacts in browning and quality of fresh-cut apples. LWT-Food Sci Technol. 2016; 66:165-71.
22. Vermeiren L, Devlieghere F, Vandekinderen I, Debevere J. The interaction of the non-bacteriocinogenic Lactobacillus sakei 10a and lactocin s producing Lactobacillus sakei 148 towards Listeria monocytogenes on a model cooked ham. Food Microbiol. 2006; 23(6):511-8.
23. Shahbazi Y, Shavisi N, Mohebi E. Effects of Ziziphora clinopodioides essential oil and nisin, both separately and in combination, to extend shelf life and control Escherichia coli O157:H7 and Staphylococcus aureus in raw beef patty during refrigerated storage. J Food Safety. 2016; 36(2):227-36.
24. Shahbazi Y, Shavisi N, Mohebi E. Potential application of Ziziphora clinopodioides essential oil and nisin as natural preservatives against Bacillus cereus and Escherichia coli O157:H7 in commercial barley soup. J Food Safety. 2016; 36(4):435-41.
25. Sebti I, Coma V. Active edible polysaccharide coating and interactions between solution coating compounds. Carbohydr Polym. 2002; 49(2):139-44.
26. Kristo E, Koutsoumanis KP, Biliaderis CG. Thermal, mechanical and water vapor barrier properties of sodium caseinate films containing antimicrobials and their inhibitory action on Listeria monocytogenes. Food Hydrocoll. 2008;22(3):373-86.
27. Singh R, Kumar M, Mittal A, Mehta PK. Microbial metabolites in nutrition, healthcare and agriculture. Biotechnol. 2017; 7(1):15-19.
28. Martinez FAC, Balciunas EM, Converti A, Cotter PD, de Souza Oliveira RP. Bacteriocin production by Bifidobacterium spp. A review. Biotechnol Advanc. 2013; 31(4):482-8.
29. Tong Z, Ni L, Ling J. Antibacterial peptide nisin: A potential role in the inhibition of oral pathogenic bacteria. Peptides. 2014; 60:32-40.
30. Al-Holy MA, Al-Nabulsi A, Osaili TM, Ayyash MM, Shaker RR. Inactivation of Listeria innocua in brined white cheese by a combination of nisin and heat. Food Control. 2012; 23(1):48-53.
31. Gyawali R, Ibrahim SA. Natural products as antimicrobial agents. Food Control. 2014; 46:412-29.
  • Receive Date: 04 May 2019
  • Revise Date: 06 August 2019
  • Accept Date: 17 August 2019
  • First Publish Date: 01 October 2019