Co- Encapsulation of Vitamin D and Calcium for Food Fortification

Document Type : Research Paper


1 Department of Food Nanotechnology, Research Institute of Food Science and Technology, PO Box: 91735-147, Mashhad, Iran

2 Advanced frying research core, Department of Food Science and Technology, Ferdowsi University of Mashhad, Mashhad, Iran


Introduction: Getting enough vitamin D and Calcium is important in keeping bones healthy and reducing the risk of developing osteopenia or osteoporosis. Methods: Two types of calcium salts and vitamin D3 were used as core materials in modified starch microcapsules. The effect of sonication process and formulation on physicochemical properties of emulsion and final spray dried powder was investigated. Results: The results suggested applying ultra-sound homogenization in 80% of maximum power for 3 min were optimum process condition to fabricate emulsions. 16 different emulsion formulations were prepared and their physicochemical properties were studied. All emulsions had narrow size distribution and more than 79% encapsulation efficiency. According to particle size and encapsulation efficiency two formulations were selected and further characterized. Both calcium carbonate and stearate containing microcapsules had more than 89% encapsulation efficiency and their particle size were 2 and 2.5 μm, respectively. 70% of vitamin D preserved during 8 month in encapsulated form. Conclusion: The results indicated that vitamin D3 and calcium co-encapsulated microcapsules can be used to prolong the stability and improve the solubility of vitamin D3 for food applications. 


  1. Abbas, S., Bashari, M., Akhtar, W., Li, W.W., Zhang, X. Process optimization of ultrasound-assisted curcumin nanoemulsions stabilized by OSA-modified starch. Ultrasonics Sonochemistry (2014).21, 1265–1274
  2. Allen, L.H., De Benoist, B., Dary, O. Hurrell, R.. W.H. Organization, Guidelines for food fortification with micronutrient. (2006)
  3. Guttoff, M.,  Saberi, A.H., McClements, D.J. Formation of vitamin D nanoemulsion-based delivery systems by spontaneous emulsification: factors affecting particle size and stability, Food Chemistry, (2015) 171, 117-122
  4. Hasanvand, E., Fathi, M., Bassiri, A., Javanmard, M., Abbaszadeh, R. Novel starch based nanocarrier for vitamin D fortification of milk: Production and characterization, Food and
  5. Bioproducts Processing, (2015). 96, 264-277
  6. Harinarayan, C. & Joshi, S.R. Vitamin D status in India–Its implications and remedial
  7. Measures (2009), 57, 40-48.
  8. Jafari, S.M., He, Y., Bhandari, B. (2007). Production of sub-micron emulsions by ultrasound and microfluidization techniques. Journal of Food Engineering 82, 478–488
  9. Karaca, A.C., Nickerson, M. & Low N.H. Microcapsule production employing chickpea or lentil protein isolates and maltodextrin: Physicochemical properties and oxidative protection of encapsulated flaxseed oil Food Chemistry (2013) 139, 448–457
  10. Kiani, A., Fathi, M., Ghasemi, S.M.  Production of novel vitamin D3 loaded lipid nanocapsules for milk fortification, International Journal of Food Properties, (2017). 20, 2466- 2476.
  11. Klinkesorn, U., Sophanodora, P., Chinachoti, P., Decker, E. A., & McClements, D. J. Characterization of spray-dried tuna oil emulsified in two-layered interfacial membranes prepared using electrostatic layer-by-layer deposition. Food Research International, (2006).  39(4), 449–457
  12. Kumssa, D.B., Joy, E.J.M., Ander, E.L., Watts, M.J., Young, S.D., Walker, S. & Broadley, M.R. Dietary calcium and zinc deficiency risks are decreasing but remain prevalent. Scientific Reports, (2015). DOI: 10.1038/srep10974
  13. Lee,J.H., O'Keefe,J.H., Bell, D. Hensrud, D.D. & Holick,M.F. Vitamin D deficiency: an important, common, and easily treatable cardiovascular risk factor?, Journal of the American
  14. College of Cardiology, (2008). 52 1949-1956.
  15. Lesmes, U., Barchechath, J., Shimoni, E., 2008. Continuous dual feed homogenization for the production of starch inclusion complexes for controlled release of nutrients. Innovative Food Science & Emerging Technologies 9, 507-515.
  16. Li, Q., Liu, C.-G., Huang, Z.-H., & Xue, F.-F. (2011). Preparation and characterization of nanoparticles based on hydrophobic alginate derivative as carriers for sustained release of vitamin D3. Journal of Agricultural and Food Chemistry, 59, 1962–1967.
  17. Luo, Y., Teng, Z., & Wang, Q. (2012). Development of zein nanoparticles coated with carboxymethyl chitosan for encapsulation and controlled release of vitamin D3. Journal of Agricultural and Food Chemistry, 60, 836–843.
  18. Maurya, V.K. & Aggarwal, M. Factors influencing the absorption of vitamin D in GIT: an overview, Journal of food science and technology, (2017).  54 3753-3765.
  19. Ozturk, B., Argin,S., Ozilgen, M., McClements, D.J. Nanoemulsion delivery systems for oilsoluble vitamins: Influence of carrier oil type on lipid digestion and vitamin D3 bioaccessibility, Food chemistry, (2015).  187 499-506.
  20. Sun-Waterhouse, D., Wadhwa, S.S., Waterhouse, G.I.N. Spray-Drying Microencapsulation of Polyphenol Bioactives: A Comparative Study Using Different Natural Fibre Polymers as Encapsulants. Food Bioprocess Technology. (2012). 2376–2388
  21. Ron, N., Zimet, P., Bargarum, J., & Livney, Y. D. (2010). Beta-lactoglobulin– polysaccharide complexes as nanovehicles for hydrophobic nutraceuticals in non-fat foods and clear beverages. International Dairy Journal, 20, 686–693.
  22. Tsiaras, W.G. & Weinstock, M.A. (2011). Factors influencing vitamin D status, Acta dermatovenereologica,91 (2011) 115-124.
  23. Winuprasith, T., Khomein, P., Mitbumrung, W., Suphantharika, M., Nitithamyong, A., McClements, D.J. 2018. Encapsulation of vitamin D3 in pickering emulsions stabilized by nanofibrillated mangosteen cellulose: Impact on in vitro digestion and bioaccessibility. Food Hydrocolloids. 83, 153-164
  1. Delavari, B., Saboury, A.A., Atri, M.S., Ghasemi, A., Bigdeli, B., Khammari,A., Maghami, P., Moosavi-Movahedi, A.A., Haertlé, T., Goliaei, B. 2015. Alpha-lactalbumin: A new carrier for vitamin D3 food enrichment. Food Hydrocolloid, 45. 124-131