The Effect of Saffron Extract Consumption Along With Aerobic Training on Glycemic Indices in Streptozotocin (STZ)-Induced Diabetic Male Wistar Rats

Document Type : Research Paper

Authors

1 Department of Exercise Physiology, South Tehran Branch, Islamic Azad University, Tehran, Iran.

2 Department of Exercise Physiology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran.

Abstract

Introduction: Diabetes, as one of the most common chronic diseases and one of the main causes. Death is known all over the world. The purpose of this study is to investigate the effect of extract consumption. Saffron combined with aerobic exercise on glycemic indices of diabetic male rats. It was with streptozotocin.  Method: The investigation method of this experimental study was carried out on 40 adult male rats with an age range of 10-12 weeks and a weight of 220-250 grams.So from the induction of diabetes in rats randomly to healthy control groups, diabetic control, Diabetic exercise, diabetic saffron and diabetic exercise + saffron group were divided. The duration of the intervention was 5 days a week and 8 weeks with moderate intensity. After some time 8 weeks of glycemic indices were measured, the data with Kolmogorov statistical tests Smirnov, one-way analysis of variance and Tukey's follow-up test were analyzed. Result: The results showed that fasting glucose in the saffron extract group with exercise Aerobics was significantly lower than the control group and lower than the aerobic training group (p=0.014). Glycosylated hemoglobin in the saffron extract group with aerobic exercise. It was lower than the control group (p=0.001) insulin resistance in the saffron consumption group. With aerobic exercise, it was lower than the control group (p=0.001). Conclusion: It is concluded that probably both an aerobic exercise course and a course Consumption of saffron extract by reducing hyperglycemic indicators can prevent diabetes to control It seems that if aerobic exercise and consumption of saffron extract at the same time used, can lead to more beneficial effects in this regard.

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Main Subjects


  1. Manna P, Das J, Ghosh J, Sil PC. Contribution of type 1 diabetes to rat liver dysfunction and cellular damage via activation of NOS, PARP, IkappaBalpha/NF-kappaB, MAPKs, and mitochondria-dependent pathways: Prophylactic role of arjunolic acid. Free Radic Biol Med. 2010 Jun 1; 48(11):1465-84. doi: 10.1016/j.freeradbiomed.2010.02.025. Epub 2010 Feb 25. PMID: 20188823.
  2. Kaltsas G, Vgontzas A, Chrousos G. Fatigue, endocrinopathies, and metabolic disorders. PM R. 2010 May; 2(5):393-8. doi: 10.1016/j.pmrj.2010.04.011. PMID: 20656620.
  3. Loscalzo J, Fauci A, Kasper D, Hauser S, Longo D, J. Jameson L. Harrison's Principles of Internal Medicine, 21e. 2011. MC Craw Hill Nedical, 18 nd Edition, Newyork.
  4. Sharifirad G, Entezari MH, Kamran A, Azadbakht L. The effectiveness of nutritional education on the knowledge of diabetic patients using the health belief model. J Res Med Sci. 2009 Jan; 14(1):1-6. PMID: 21772854; PMCID: PMC3129063.
  5. D, Anderson-Jackson L, V. T. Biochemical Evaluation of Oxidative Stress in Type 1 Diabetes [Internet]. Type 1 Diabetes. InTech; 2013. Available from: http:// dx.doi. org/ 10.5772/52836.
  6. https://www.who.int/news-room/fact-sheets/detail/diabetes.
  7. Guyton and Hall Textbook of Medical Physiology (Guyton Physiology) 13th Edition.2015.
  8. Noormohammadpour P, Halabchi F, Mazaheri R, Mansournia MA, Alizadeh Z, Seif Barghi T, Abolhasani M, Kordi R. Designing and implementing a curriculum for Sports and Exercise Medicine elective course for undergraduate medical students of Tehran University of Medical Sciences. Br J Sports Med. 2019 May; 53(10):601-604. doi: 10.1136/bjsports-2018-099462. Epub 2018 Jun 22. PMID: 29934428.
  9. Amin, G.R. Popular Medicinal Plants of Iran. Iranian Research Institute of Medicinal Plants, Tehran, 1-66.1991. (In Persian).
  10. McLennan SV, Heffernan S, Wright L, Rae C, Fisher E, Yue DK, Turtle JR. Changes in hepatic glutathione metabolism in diabetes. Diabetes. 1991 Mar;40(3):344-8. doi: 10.2337/diab.40.3.344. PMID: 1671844.
  11. Daglu A. The secrets of plants. Tehran: Chehar Publications; 1984.
  12. Yeh GY, Eisenberg DM, Kaptchuk TJ, Phillips RS. Systematic review of herbs and dietary supplements for glycemic control in diabetes. Diabetes Care. 2003 Apr;26(4):1277-94. doi: 10.2337/diacare.26.4.1277. PMID: 12663610.
  13. Kianbakht S. A Systematic Review on Pharmacology of Saffron and its Active Constituents. J. Med. Plants 2008; 7 (28) :1-27.URL: http://jmp.ir/article-1-402-en.html.
  14. Li SC, Liu YH, Liu JF, Chang WH, Chen CM, Chen CY. Almond consumption improved glycemic control and lipid profiles in patients with type 2 diabetes mellitus. Metabolism. 2011 Apr; 60(4):474-9. doi: 10.1016/j.metabol.2010.04.009. Epub 2010 May 23. PMID: 20580779.
  15. Goguen JM, Leiter LA. Lipids and diabetes mellitus: a review of therapeutic options. Curr Med Res Opin. 2002; 18 Suppl 1:s58-74. doi: 10.1185/030079902125000246. PMID: 12365820.
  16. Skourtis G, Krontira A, Ntaoula S, Ferlemi AV, Zeliou K, Georgakopoulos C, Margarity GM, Lamari NF, Pharmakakis N. Protective antioxidant effects of saffron extract on retinas of streptozotocin-induced diabetic rats. Romanian journal of ophthalmology. 2020 Oct;64(4):394.
  17. Sani A, Tajik A, Seiiedi SS, Khadem R, Tootooni H, Taherynejad M, Sabet Eqlidi N, Alavi dana SM, Deravi N. A review of the anti-diabetic potential of saffron. Nutrition and Metabolic Insights. 2022 Jul; 15:11786388221095223.
  18. hooshmand moghadam B, Rashid lamir A, Attarzade Hosseini S R, Gaeini A A. A Review on the Effects of Saffron with Physical Activity. J. Med. Plants 2019; 18 (72) :14-29
  19. Vahid Aghajani, Marzieh Nazari, Ramin Shabani. [Impact of aerobic and resistance training supplemented with the consumption of saffron on glutathione peroxidase and malondialdehyde in men with type 2 diabetes]. J Gorgan Univ Med Sci. 2019 autumn; 21(3): 24-33. [In Persian].
  20. Trinder P. Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen. J Clin Pathol. 1969 Mar;22(2):158-61. doi: 10.1136/jcp.22.2.158. PMID: 5776547; PMCID: PMC474026.
  21. Xi L, Qian Z, Shen X, Wen N, Zhang Y. Crocetin prevents dexamethasone-induced insulin resistance in rats. Planta Med. 2005 Oct;71(10):917-22. doi: 10.1055/s-2005-871248. PMID: 16254822.
  22. Asai A, Nakano T, Takahashi M, Nagao A. Orally administered crocetin and crocins are absorbed into blood plasma as crocetin and its glucuronide conjugates in mice. J Agric Food Chem. 2005 Sep 7; 53(18):7302-6. doi: 10.1021/jf0509355. PMID: 16131146.
  23. Shavandi N, Sharjerdi S, Sheikh Hoseini R, Ghorbani A. The Effect of Strengthening Exercises on Metabolic Factors, Quality of Life and Mental Health in Women with Type 2 Diabetes. Iranian Journal of Endocrinology and Metabolism 2010; 12 (3):222-230.URL: http://ijem.sbmu.ac.ir/article-1-836-en.html.
  24. Tadibi V, Bayat Z. [Effect of eight weeks aerobic training and drug intervention on quality of life in women with type 2 diabetes]. J Gorgan Uni Med Sci. 2012; 14(2): 30-36. [In Persian].
  25. Winnick JJ, Sherman WM, Habash DL, Stout MB, Failla ML, Belury MA, Schuster DP. Short-term aerobic exercise training in obese humans with type 2 diabetes mellitus improves whole-body insulin sensitivity through gains in peripheral, not hepatic insulin sensitivity. J Clin Endocrinol Metab. 2008 Mar; 93(3):771-8. doi: 10.1210/jc.2007-1524. Epub 2007 Dec 11. PMID: 18073312; PMCID: PMC2266960.
  26. Park S, Hong SM, Lee JE, Sung SR. Exercise improves glucose homeostasis that has been impaired by a high-fat diet by potentiating pancreatic beta-cell function and mass through IRS2 in diabetic rats. J Appl Physiol (1985). 2007 Nov;103(5):1764-71. doi: 10.1152/japplphysiol.00434.2007. Epub 2007 Aug 30. PMID: 17761790.
  27. Yavari A, Najafipoor F, Aliasgarzadeh A, Niafar M, Mobasseri M, Nikookheslat S. Effect of Aerobic Exercise, Resistance Training or Combined Training on Glycemic Control and Cardiovascular Risk Factors in Patients with Type 2 Diabetes Mellitus. Journal Tabriz Medicine Sciences. 2011; 33(4): 82-91.
  28. Hajihasani A, Bakhtiari A H, Hedayati R. Effects of eccentric and concentric exercises on blood pressure and heart rate parameters in type 2 diabetes. Koomesh 1393; 16 (2):166-174
    URL: http://koomeshjournal.semums.ac.ir/article-1-2340-en.html.
  29. Dunstan DW, Vulikh E, Owen N, Jolley D, Shaw J, Zimmet P. Community center-based resistance training for the maintenance of glycemic control in adults with type 2 diabetes. Diabetes Care. 2006 Dec;29(12):2586-91. doi: 10.2337/dc06-1310. PMID: 17130189.
  30. Fenicchia LM, Kanaley JA, Azevedo JL Jr, Miller CS, Weinstock RS, Carhart RL, Ploutz-Snyder LL. Influence of resistance exercise training on glucose control in women with type 2 diabetes. Metabolism. 2004 Mar;53(3):284-9. doi: 10.1016/j.metabol.2003.10.007. PMID: 15015138.
  31. Hazman Ö, Ovalı S. Investigation of the anti-inflammatory effects of safranal on high-fat diet and multiple low-dose streptozotocin induced type 2 diabetes rat model. Inflammation. 2015 Jun;38:1012-9.
  32. Tajaddini A, Roshanravan N, Mobasseri M, Haleem Al‐qaim Z, Hadi A, Aeinehchi A, Sefid‐Mooye Azar P, Ostadrahimi A. The effect of saffron (Crocus sativus L.) on glycemia, lipid profile, and antioxidant status in patients with type‐2 diabetes mellitus: A randomized placebo‐controlled trial. Phytotherapy Research. 2023 Feb;37(2):388-98.
  33. Kang C, Lee H, Jung ES, Seyedian R, Jo M, Kim J, Kim JS, Kim E. Saffron (Crocus sativus L.) increases glucose uptake and insulin sensitivity in muscle cells via multipathway mechanisms. Food chemistry. 2012 Dec 15;135(4):2350-8.
  34. Rajabi A, Khajehlandi M, Siahkuhian M, Akbarnejad A, Khoramipour K, Suzuki K. Effect of 8 weeks aerobic training and saffron supplementation on inflammation and metabolism in middle-aged obese women with type 2 diabetes mellitus. Sports. 2022 Oct 30;10(11):167.
Volume 12, Issue 1
March 2024
Pages 36-41
  • Receive Date: 23 September 2023
  • Revise Date: 24 December 2023
  • Accept Date: 25 December 2023